Universidade de Vigo

Description of the legal entity :

The University of Vigo (UVigo, www.uvigo.es) has placed a considerable emphasis on R&D activities by way of numerous internal funded projects, as well as through its various services and research centres. This support has enormously increased the scientific output and the capacity to obtain external resources. UVigo participates every year in the FP and other EU initiatives; more than 50 EU Projects has been developed since 2007, with a return of >25M €. The CINBIO (www.cinbio.es) is a member of the Singular Research Centres Network funded by Galician Government and one of the most outstanding centers at UVigo, carrying out an externally recognized and strongly supported research activity.

Isabel Pastoriza-Santos (F) (Coordinator)) (ORCID: 0000-0002-1091-1364).

She is currently Assoc. Prof at CINBIO and Department of Physical Chemistry at Universidade de Vigo. Since 2012, she heads the Functional Nanobiomaterials Group (formerly Colloid Chemistry Group), a multidisciplinary team (chemists, biologists, and physicists) form by 3 PIs, 5 research fellows and postdocs, 5 PhD students, 1 Technician and 1 Project manager. The research of FunNanobio focuses on multiple aspects of nanoscience and plasmonics, including the synthesis of functional nanostructured materials, experimental and theoretical study of plasmonic properties. FunNanobio group has contributed to the development of platforms for ultrasensitive SERS detection and bioimaging. The BIOCELLPHE team includes Dr. Pérez-Juste (Assoc. Prof., plasmonics and SERS; ORCID: 0000-0002-4614-1699), Dr. Bodelón (Research Fellow, microbiology) Sara Núñez-Sánchez (Research Fellow, plasmonics; ORCID: 0000-0002-5435-6892) and Daniel García-Lojo (PhD student, nanoparticles self-assembly and SERS; ORCID: 0000-0002-5477-6299) and Mª José Cordero (technician, general experimental support).

Role in the project :

Synthetic bacterial sensor scaffolds and PS testing the biosynthetic RaR by SERS.

Transenvelope signalling in the multiplex SERS validation of bacterial bioreporters

Generation of biosynthetic pathways for RaR, performing the SERS analysis

Fabricating plasmonic platform with outstanding SERS performance

Fabrication of LoC- SERS device and validation

The UVigo will intensively participate in dissemination, communication and exploitation

The UVigo will lead the project

Infrastructure and facilities :

The UVigo team has fully access to cutting-edge technologies in the field of plasmonics and colloid chemistry with 100 m2 of laboratory space and additional office for graduate students and postdocs.

The lab space is divided in three labs: chemistry, a biology and a instrumentation lab. Its infrastructure includes different spectroscopic techniques (absorption, fluorescence, Raman), DLS, soft-lithography station, etc. In addition, the CINBIO is fully equipped with general cellular and microbiology facilities including a BSL3 laboratory.

Finally, the team is supported by the general facilities at UVigo (TEM, SEM, AFM, Confocal, sequencer, MS, etc.).

Related projects and publications :

Microfluidic-induced hierarchical 3D assemblies of metal nanoparticles for advanced catalytic and sensing applications (MAT2016-77809R).

PLASMAQUO, Nanochemistry development of plasmonic quorum sensors for understanding bacterial-eukaryotic cell relations (H2020-ERC- ADG-267867). SERSing, Advanced Surface Enhanced Raman Spectroscopy (SERS) based technologies for gas and liquids sensing in the area of chemical protection (H2020-EU.3.7.5.-SU-DRS04-2019-2020).

SERSforSAFETY, Development of LOC nanostructured sensors for the detection of pathogens and food- borne chemical agents based on SERS. FOODSENSE (INTERREG V-A España-Portugal 2014-2020 (POCTEP)). SERSING

 

LIST OF 5 PUBLICATIONS.

  • Castro-Grijalba A., et al. SERS-based molecularly imprinted plasmonic sensor for highly sensitive PAH detection, ACS Sens. 2020, 5, 3, 693-702.
  • Bodelón G, et al. Imaging Bacterial Inter-species chemical interactions by SERS. ACS Nano, 2017, 11, 4631.
  • Bodelón G, et al. Detection and imaging of QS in P aeruginosa biofilm communities by SERS. Nature Mater. 2016, 15, 1203.
  • Gómez-Graña S, et al. Gold nanooctahedra with tunable size and microfluidic-induced 3D assembly for highly uniform SERS-active supercrystals. Chem. Mater. 2015, 27, 8310.
  • Bodelón G, et al. Au@pNIPAM SERRS tags for multiplex immunophenotyping cellular receptors and imaging tumor cells. Small 2015, 11, 4149.
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