PUBLICATIONS
2025
25. Alexandre Chícharo, Alexandra Teixeira, Diogo Moreira, Maria S. Relvas, Marta Aranda,Temple Douglas, Jerôme Borme, Jana B. Nieder, Lorena Diéguez, and Sara Abalde-Cela, “Precision-Engineered Plasmonic Nanostar Arrays forHigh-Performance SERS Sensing“, Advanced Optical Materials, 2025. [Link]
24. Adriana Carneiro, Marta Aranda Palomer, Margarida Esteves, Carolina Rodrigues,José Maria Fernandes, Francisca Oliveira, Alexandra Teixeira, Carlos Honrado,Lorena Dieguez, Sara Abalde-Cela, and Miguel Xavier, “Advanced Microfluidics for Single Cell-Based Cancer Research“, Advanced Science, 2025. [Link]
23. Adrián Fernández-Lodeiro, Carlos Fernández-Lodeiro, Silvia Nuti, Ignacio Pérez-Juste, Isabel Pastoriza-Santos, Jorge Pérez-Juste, Enrique Carbo-Argibay, José Luis Capelo-Martínez, Carlos Lodeiro and Javier Fernández-Lodeiro, “Precise control of silver nanoplate dimensions and optical properties via pH, EDTA and AMP mediated synthesis“, Journal of Colloid And Interface Science, 2025. [Link]
2024
22. Julien Capin, Emile Chabert, Ana Zuñiga and Jerome Bonnet, “Microbial biosensors for diagnostics, surveillance and epidemiology: Today’s achievements and tomorrow’s prospects“, Microbial Biotechnology, 2024. [Link]
21. Claire Hotton, Daniel García-Lojo, Evgeny Modin, Rahul Nag, Sergio Gómez-Graña,Jules Marcone, Jaime Gabriel Trazo, Jennifer Bodin, Claire Goldmann, Thomas Bizien,Isabel Pastoriza-Santos, Brigitte Pansu, Jorge Pérez-Juste, Victor Balédent, and Cyrille Hamon, “Elucidating Supercrystal Mechanics and Nanoparticle Sizeand Shape Effects under High Pressure“, Small Structures, 2024. [Link]
20. Daniel Garcia-Lojo, Sergio Rodal-Cedeira, Sara Nunez-Sanchez, Daniel Arenas-Esteban, Lakshminarayana Polavarapu, Sara Bals, Jorge Perez-Juste, and Isabel Pastoriza-Santos, “Pentatwinned AuAg Nanorattles with Tailored Plasmonic Properties for Near-Infrared Applications”, Chemistry of Materials, 2024. [Link]
19. Krishna Kant, Reshma Beeram, Yi Cao, Paulo S. S. dos Santos, Lara Gonzalez-Cabaleiro, Daniel Garcia-Lojo, Heng Guo, Younju Joung, Siddhant Kothadiya, Marta Lafuente, Yong Xiang Leong, Yiyi Liu, Yuxiong Liu, Sree Satya Bharati Moram, Sanje Mahasivam, Sonia Maniappan, Daniel Quesada-Gonzalez, Divakar Raj, Pabudi Weerathunge, Xinyue Xia, Qian Yu, Sara Abalde-Cela, Ramon A. Alvarez-Puebla, Rizia Bardhan, Vipul Bansal, Jaebum Choo, Luis C. C. Coelho, Jose M. M. M. de Almeida, Sergop Gomez-Grana, Marek Grzelczak, Pablo Herves, Jatish Kumar, Theobald Lohmueller, Arben Merkoci, Jose Luis Montano-Preide, Xing Yi Ling, Reyes Mallada, Jorge Perez-Juste, Maria P. Pina, Srikanth Singamaneni, Venugopal Rao Soma, Mengtao Sun, Limei Tian, Jianfang Wang, Lakshminarayana Polavarapu, and Isabel Pastoriza Santos, “Plasmonic nanoparticle sensors: current progress, challenges, and future prospects”, Nanoscale Horizons, 2024. [Link]
18. Soraia Lobo-Martins, Patrícia Corredeira, Ana Cavaco, Carolina Rodrigues, Paulina Piairo, Cláudia Lopes, Joana Fraga, Madalena Silva, Patrícia Alves, Lisiana Wachholz Szeneszi, Ana Barradas, Camila Castro Duran, Marília Antunes, Gonçalo Nogueira-Costa, Rita Sousa, Conceição Pinto, Leonor Ribeiro, Catarina Abreu, Sofia Torres, António Quintela, Gadea Mata, Diego Megías, Julie Ribot, Karine Serre, Sandra Casimiro, Bruno Silva-Santos, Lorena Diéguez, and Luís Costa,, “Effect of Cyclin-Dependent Kinase 4/6 Inhibitors on Circulating Cells in Patients with Metastatic Breast Cancer“, Cells, 2024. [Link]
17. Lara Gonzalez-Cabaleiro, Carlos Fernandez-Lodeiro, Lorena Vazquez-Iglesias, Pablo Soriano-Maldonado, Mark J. van Raaij, Gustavo Bodelon, Jorge Perez-Juste, and Isabel Pastoriza-Santos, “Pushing the Limits of Lateral Flow Immunoassay by Digital SERS for the Ultraflow Detection of SARS-CoV-2 Virus“, Small Science, 2024. [Link]
16. Alejandro Prieto, Luïsa Miró, Yago Margolles, Manuel Bernabeu, David Salguero, Susana Merino, Joan Tomas, Juan Alberto Corbera, Anna Perez-Bosque, Mario Huttener, Luis Ángel Fernandez and Antonio Juarez, “Targeting plasmid-encoded proteins that contain immunoglobulin-like domains to combat antimicrobial resistance“, eLife, 2024. [Link]
15. Ecem Tiryaki, Carla Á lvarez-Leirós, Julia N. Majcherkiewicz, Paul L. Chariou, Melodie Maceira-Campos,Gustavo Bodelón, Nicole F. Steinmetz, and Verónica Salgueiriñ, “Magnetically Induced Thermal Effects on Tobacco Mosaic Virus-Based Nanocomposites for a Programmed Disassembly of Protein Cages“, Applied Bio Materials, 2024. [Link]
14. Alba Vazquez-Arias, Lorena Vazquez-Iglesias, Ignacio Perez-Juste, Isabel Pastoriza-Santos, and Gustabo Bodelon, “Bacterial surface display of human lectins in Escherichia coli“, Microbial Biotechnology, 2024 [Link]
13. Lorena Vazquez-Iglesias, Giovanna Maria Stanfoca Casagrande, Daniel Garcia-Lojo, Leticia Ferro Leal, Tien Anh Ngo, Jorge Perez-Juste, Rui Manuel Reis, Krishna Kant, and Isabel Pastoriza-Santos, “SERS sensing for cancer biomarker: Approaches and directions”, Bioactive Materials, 2024. [Link]
2023
12. Carla Estévez-Varela, Sara Núñez-Sánchez,* Paula Piñeiro-Varela, Dorleta Jiménez deAberasturi, Luis M. Liz-Marzán, Jorge Pérez-Juste, and Isabel Pastoriza-Santos, “Plexcitonic Nanorattles as Highly Efficient SERS-Encoded Tags“, Small, 2023. [Link]
11. Ecem Tiryaki, Saida Ortolano, Gustavo Bodelon and Veronica Salgueirino, “Programming an Enhanced Update and the Intracellular Fate of Magnetic Microbeads“, Advanced Healthcare Materials, 2023. [Link]
10. Carlos Fernandez-Lodeiro, Lara Gonzalez-Cabaleiro, Lorena Vazquez-Iglesias, Esther Serrano-Pertierra, Gustavo Bodelon, Monica Carrera, Maria Carmen Blanco-Lopez, Jorge Perez-Juste and Isabel Pastoriza-Santos, “Au@Ag Core-Shell Nanoparticles for Colorimetric and Surface-Enhanced Raman-Scattering-Based Multiplex Competitive Lateral Flow Immunoassay for the Simultaneous detection of Histamine and Parvalbumin in Fish“, ACS Applied Nano Materials, 2023. [Link]
9. Lidia Cerdán, Beatriz Álvarez and Luis Ángel Fernández, “Massive integration of large gene libraries in the chromosome of Escherichia coli“, Microbial Biotechnology, 2023. [Link]
8. Alejandro Asensio-Calavia, Álvaro Ceballos-Munuera, Almudena Méndez-Pérez, Beatriz Álvarez and Luis Ángel Fernández, “A Tuneable genetic switch for tight control of tac promoters in Escherichia coli boosts exopression of synthetic injectosomes“, Microbial Biotechnology, 2023. [Link]
7. Carlos Fernandez-Lodeiro, Javier Fernandez-Lodeiro, Adrian Fernandez-Lodeiro, Silvia Nuti, Carlos Lodeiro, Alec LaGrow, Ignacio Perez-Juste, Jorge Perez-Juste and Isabel Pastoriza-Santos, “Synthesis of tuneable gold nanostars: the role of adenosine monophosphate”, Journal of Materials Chemistry C, 2023. [Link]
2022
6. Kevin Oliveira, Alexandra Teixeira, Jose M. Fernandes, Claudia Lopes, Alexandre Chicharo, Paulina Piairo, Lei Wu, Laura Rodriguez-Lorenzo, Lorena Dieguez and Sara Abalde-Cela, “Multiplex SERS Phenotyping of Single Cancer Cells in Microdroplets”, Advanced Optical Materials, 2022. [Link]
5. Beatriz Álvarez, Victor Muñoz-Abad, Alejandro Asensio-Calavia and Luis Ángel Fernandez, “Enhanced protein translocation to mammalian cells by expression of EtgA transglycosylase in a synthetic injector E. coli strain”, Microbial Cell Factories, 2022. [Link]
4. Rosana S. Molina, Gordon Rix, Amanuella A. Mengiste, Beatriz Álvarez, Daeje Seo, Haiqi Chen, Juan E. Hurtado, Qiong Zhang, Jorge Donato García-García, Zachary J. Heins, Patrick J. Almhjell, Frances H. Arnold, Ahmad S. Khalil, Andrew D. Hanson, John E. Dueber, David V. Schaffer, Fei Chen, Seokhee Kim, Luis Ángel Fernández, Matthew D. Shoulders and Chang C. Liu,’ In vivo hypermutation and continuous evolution‘, Nature Reviews Methods Primers, 2022. [Link]
3. Daniel García-Lojo,David Méndez-Merino,Ignacio Pérez-Juste, Ángel Acuña,Luís García-Río,Alfonso Rodríguez-Patón,Isabel Pastoriza-Santos,Jorge Pérez-Juste, “Bolaform Surfactant-Induced Au Nanoparticle Assemblies for Reliable Solution-Based Surface-Enhanced Raman Scattering Detection”, Advanced Materials Technologies, 2022. [Link]
2021
2. Elena M. Seco and Luiz Ángel Fernandez, “Efficient markerless integration of genes in the chromosome of probiotic E. coli Nissle 1917 by bacterial conjugation“, Microbial Biotechnology, 2021. [Link]
1. Yamal Al-ramahi, Akos Nyerges, Yago Margolles, Lidia Cerdan, Gyorgyi Ferenc, Csaba Pal, Luis Ángel Fernandez and Victor de Lorenzo, “ssDNA recombineering boosts in vivo evolution of nanobodies displayed on bacterial surfaces“, Communications Biology, 2021. [Link]
THESES
MASTER THESIS Diego Crespo Roche, ‘Optimization of the in vivo mutagenesis system T7-DIVA for directed evolution of proteins‘, Universidad Autónoma de Madrid. Supervised by Luis-Angel Fernandez and Beatriz Álvarez (CSIC), 7th July 2022.
PhD THESIS Álvaro Ceballos Munuera, ‘Development of genetic circuits and synthetic receptors in Escherichia coli for detecting tumour-associated metabolites and protein antigens‘, Universidad Autónoma de Madrid. Supervised by Luis-Angel Fernandez (CSIC), 29th June 2023. [PDF]
MASTER THESIS Ana Ferrández Múrtula, ‘Engineering E. coli for targeted tumour cell invasion and tozin delivery‘, Universidad Internacional Menendez Pelayo. Supervised by Luis-Angel Fernandez and Elena María Seco (CSIC), 14th June 2024.
MASTER THESIS Pablo Becana Minguez, ‘Optimizations of the T7-DIVA in vivo mutagenesis system for protein molecular evolution’, Universidad Autónoma de Madrid. Supervised by Luis-Angel Fernandez and Beatriz Álvarez (CSIC), 28th June 2024.
PhD THESIS Álba Cabrera Fisac, ‘Evaluation of genetically modified Escherichia coli bacteria in 3D models of tumor spheroids for the development of treatments against solid epithelial tumors’, Universidad Autónoma de Madrid. Supervised by Luis-Angel Fernandez and Elena María Seco (CSIC), 16th January 2025.
MASTER THESIS Lucía Hontallina Yedra , ‘Characterization of cell death in colorectal cancer cells by cytotoxin injection with genetically modified Escherichia coli strains’, Universidad Autónoma de Madrid. Supervised by Luis-Angel Fernandez and Eva Pico Sánchez (CSIC), 25th June 2025.
MASTER THESIS Morgan Climent, ‘Optimizing the bioproduction of violacein’. Supervised by Jean-Loup Fallon (INRAe), 2025.
PATENTS
1. “Chimeric Proteins and their use as an antigen detecting system” submitted by CSIC under reference no: EP23382158 on 21st February 2023. Filed as an International PCT under reference no: PCT/EP2024/054448 on 21st February 2024. Officially published on 28th August 2024.
POSTERS
9. “Highly ordered nanostars-based solid substrate for ultrasensitive SERS” presented by Alexandre Chicharo, INL, at the International Conference on Advanced Vibrational Spectroscopy (ICAVS12), 27th August – 1st September 2023 in Krakow, Poland. [Link]
8. “Integration of a SERS solid substrate with microdroplet technology for cancer metabolite detection” presented by Alexandre Chicharo, INL, at the 6th Annual Meeting of CINBIO, 20-21st July 2023 in Vigo, Spain. [Link]
7. “Microfluidics for Cancer Research” presented by Miguel Xavier, INL, at Gordon Research Conferences – Physics and Chemistry of Microfluidics, 4-9th June 2023 in Lucca, Italy. [Link]
6. “Highly-sensitive nanostar SERS substrate for cancer metabolite detection” presented by Alexandre Chicharo, INL, at Nffa.eu Pilot Training School, 27-28th September 2022 in Braga, Portugal. [Link]
5. “A synthetic trans-envelope signalling system for multiplexed protein detection in E. coli” presented by Julien Capin, INSERM, at Symposium on Synthetic and Systems Biology, 12-14th September 2022 in Paris, France. [Link]
4. “Engineered E.coli to produce Raman Reporters (RaRs) that can be detected by Surface-enhanced Raman Scattering (SERS)” presented by Hadi Jbara, INRAE, at Symposium on Synthetic and Systems Biology, 12-14th September 2022 in Paris, France. [Link]
3. “SERS Nanostar substrate for cancer metabolite detection” presented by Alexandre Chicharo, INL, at Sustainable Nanofabrication Joint Networking Event, 5-7th July 2022 in Braga, Portugal. [Link]
2. “Ultrasensitive nanostar substrate for cancer metabolite detection using Surface-enhanced Raman Scattering” presented by Alexandre Chicharo, INL, at ETPN2022 – 7th Annual event of European Technology Platform on Nanomedicine (ETPN), 22nd June 2022 in Braga, Portugal. [Link]
1. “Combination of nanolithography and wet chemistry for the fabrication of an ultrasensitive SERS nanostars array applied to cancer metabolite detection” presented by Alexandre Chicharo, INL, at SPIE Photonics Europe 2022, 6th April 2022 in Strasbourg, France. [Link]